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Why a shot
Swallowing a nutrient is not the same as absorbing it. An intramuscular injection removes the gut from the equation entirely, which matters far more often than most people realise.
Stomach acid, intrinsic factor, an intact terminal ileum — oral B12 needs all three. An injection needs none of them.
No line, no chair time. Most people are back out the door in the time an infusion takes to set up.
This is an MD-led clinic, not a self-serve menu. Dose and interval are set for you, and we can requisition the bloodwork behind them.
Add a shot to an infusion appointment, or run a short course of five between visits. Every shot is priced both ways.
Candidacy
Shots are a good tool for a specific set of problems and a poor substitute for a diagnosis. Here is where we would and would not start you here.
Not sure which side you fall on? Book a wellness intake and we will tell you plainly — including when the answer is that a shot is not what you need.
The menu, explained
One nutrient, one job. Below is what each is used for and how strong the evidence behind it is — including where it is thin, because you should know that before you pay for it.
5,000 mcg or 1,000 mcg · IM
For confirmed or likely B12 deficiency: fatigue that sleep does not fix, brain fog, pins and needles, a sore tongue, low mood. Also for anyone whose absorption is compromised by medication, surgery or bowel disease.
Evidence: strong for correcting deficiency. Cochrane found oral and intramuscular B12 normalise serum levels similarly in the trials available — but those were three small studies, none of which measured symptoms, and injection remains the reliable route when absorption is the problem.
More on B12 shots in Toronto: dosing, pricing and who they suit
100,000 IU · IM · Bloodwork required
A single depot dose for confirmed deficiency, particularly where daily oral dosing has not held levels or adherence has been difficult. We confirm on recent labs before giving it.
Evidence: good. Head-to-head trials show a single high-dose intramuscular injection raises and sustains 25(OH)D comparably to oral regimens, with a slower, flatter curve.
Standard B-100 formulation · IM
The full B group together rather than B12 alone — used where diet, alcohol intake, malabsorption or sustained stress make a broad B-vitamin shortfall likely.
Evidence: strong for correcting measured deficiency; modest and non-specific in people who are already replete.
600 mg · IV push
The body's principal intracellular antioxidant, and a direct regulator of the pigment pathway. Used here for oxidative-stress support — often alongside ozone or detoxification protocols — and for skin.
Evidence: randomised trials of oral glutathione have shown a measurable fall in melanin index, and topical formulations improve skin brightness over a matter of weeks. Separately, intravenous glutathione has outperformed oral N-acetylcysteine at preventing contrast-related renal oxidative stress.
Glutathione · Vitamin C · Zinc · IV push
A short immune-support push, most often used at the front end of a cold or through a heavy travel or exposure period.
Evidence: modest. Regular vitamin C does not stop most adults catching colds; it does shorten them slightly. Treat this as support, not prevention.
10 mg · IM
Requested for hair, skin and nails. Genuinely useful where biotin is low — which is uncommon, but real after bariatric surgery, on long-term anticonvulsants, or with certain genetic conditions.
Evidence: weak outside deficiency. A 2026 systematic review found no consistent benefit for hair growth without a documented shortfall. Note too that biotin interferes with common lab immunoassays — time it away from bloodwork.
Methionine · Inositol · Choline + B12 · IM
Methionine, inositol and choline together with B12, given intramuscularly. Requested most often as part of a broader metabolic or weight programme.
Evidence: there are no trials on this specific combination given as an injection, so we will not claim one. What each component does on its own is better established. Choline is required to package and export fat out of the liver — deficiency causes fat to accumulate there. Methionine is an essential amino acid and the precursor to SAMe, the body's principal methyl donor. Inositol participates in insulin signalling, with the strongest trial evidence in PCOS. B12 is a cofactor for methylation and for energy metabolism.
10 / 50 / 100 / 200 mg · IM · Titrated
Energy, mental clarity and recovery are what people come to us for. A short injection instead of a long infusion, and a sensible way to trial NAD+ before committing to an IV course.
Evidence: active and promising rather than settled. NAD+ precursors reliably raise NAD+ levels in humans; what that translates to clinically is still being worked out. We start everyone at 10 mg and titrate.
More on NAD+ injections: titration, pricing and what to expect
Every shot is available as a single dose or a five-pack. Prices include administration by our clinical staff. Vitamin D3 at 100,000 IU is given only when recent bloodwork confirms a deficiency.
NAD+ IM can feel intense at higher doses. We start patients at 10mg and titrate up only as tolerated — the same approach Dr. Kostovic uses for the IV protocol. This isn't a self-selected menu.
The evidence
We would rather you read the research than take our word for it. Every source below is peer-reviewed and linked in full. Where the evidence is thin, we have said so.
Cochrane's review of oral versus intramuscular B12 found both routes normalised serum B12 similarly — on low-quality evidence from three small trials, none of which reported symptoms. Injection remains the dependable route where intrinsic factor, stomach acid or the terminal ileum are the limiting factor.
Metformin and proton pump inhibitors each reduce B12 absorption, and the risk compounds when they are taken together — a pattern seen repeatedly in people managing type 2 diabetes and reflux at the same time.
Head-to-head trials in adults with deficiency show one high-dose intramuscular injection of cholecalciferol raises and sustains 25(OH)D comparably to daily or weekly oral dosing, with a slower and flatter release curve.
NAD+ precursors reliably raise NAD+ levels in humans, and pharmacokinetic work on intravenous NAD+ shows how quickly the body takes it up and metabolises it — which is exactly why infusion rate, and dose titration, matter so much. What those level changes mean clinically is an active question, not a closed one.
MTHFR is the enzyme that produces the active folate driving methylation. Its C677T variant is not exotic: roughly four in ten people carry one copy and about one in ten carry two. Two copies reduce enzyme activity by around seventy per cent, one copy by about a third, and homocysteine rises accordingly.
People carrying it tend to arrive describing the same cluster: fatigue that rest does not fix, brain fog, low mood, and gut symptoms. That cluster is what prompts us to test; it is not a diagnosis in itself, and plenty of other things produce it. Where a variant is present, B12 status matters more, because methylcobalamin is the cofactor the body uses to clear homocysteine with the folate MTHFR produces. We use methylcobalamin, a natural form of B12, rather than cyanocobalamin, a synthetic form whose cyanide group the body has to remove first. A 2017 review of B12 forms favoured the natural forms for bioavailability and safety.
Biotin has no consistent effect on hair growth without a documented deficiency — a 2026 systematic review found no benefit from supplementation absent a proven shortfall. Vitamin C does not prevent colds in the general population, though it shortens them modestly. We would rather you knew that here than found out afterwards.
What to expect
Four steps, and the first one is short. We are not going to put you through a full workup to give you a B12 shot — but we are also not going to inject you without knowing who you are.
Step one
A short intake covering your history, medications, supplements and what you are actually trying to fix. You get portal access when you book, so any recent bloodwork or records can be uploaded before you arrive.
Step two
Our clinical team sets the shot, the dose and the interval. If your labs point somewhere else — iron, thyroid, something that needs a proper consultation — we will say so rather than sell you an injection. Vitamin D3 is only given once recent bloodwork confirms a deficiency.
Step three
The injection itself takes moments and is given by our clinical staff under physician oversight. IV pushes take a few minutes longer. You can drive yourself home and go straight back to your day.
Step four
Most shots are offered as a five-pack because most are worth repeating on a set interval rather than once. Where it is useful, we recheck levels and adjust — and we are happy to send results to your family doctor to keep your care joined up.
Contact Us
Locations
Upper Room Clinic – Oakville
Tel: 647-521-8024 ext. 2
Fax: (289) 644-0255
oakville@upperroomclinic.com
Upper Room Clinic – Davisville
Tel: 647-521-8024
Fax: 647-348-8024
hello@upperroomclinic.com
Tuesday to Saturday
11am-6pm
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